Cell-free supernatants of lactobacilli inhibit methicilin-resistant staphylococcus aureus, vancomycin-resistant enterococcus and carbapenem-resistant klebsiella strains

Aim: Antibiotic resistance is a major health problem. Recently, probiotics are used in the field of alternative/supportive medicine. Thus, this study, we aimed to evaluate vitro inhibitory effects four different cell-free supernatants (CFSs) lactobacilli species (L. fermentum, L. plantarum, acidophilus and rhamnosus) on clinically isolated Methicilin-resistant Staphylococcus aureus (MRSA) strains, Vancomycin-resistant Enterococcus (VRE) Carbapenem-resistant Klebsiella (CRK) strains. Materials Methods: Lactobacillus strains were grown de Man Rogosa Sharpe broth; after filtration, CFSs diluted obtain 25, 50, 100% concentrations. Pathogen bacteria tryptic soy broth with without micro-plate. The bacterial growths measured using spectrophotometric method hours incubation at 37°C. One-way ANOVA followed by Dunnett’s multiple comparisons Kruskal-Wallis test for statistical analyses. Results: All tested all concentrations found inhibit growth MRSA, VRE, CRK strains; results statistically significant (p<0.0001). At 50% concentrations, be most effective MRSA growth. L.fermentum, L.acidophilus L.plantarum concentration VRE L.fermentum CRK. Conclusion: In our support that multi-drug resistant bacteria. Their dependent microorganisms CFS